Get tested with Superpower

If you’ve been postponing blood testing for years or feel frustrated by doctor appointments and limited lab panels, you are not alone. Standard healthcare is often reactive, focusing on testing only after symptoms appear or leaving patients in the dark.

Superpower flips that approach. We give you full insight into your body with over 100 biomarkers, personalized action plans, long-term tracking, and answers to your questions, so you can stay ahead of any health issues.

With physician-reviewed results, CLIA-certified labs, and the option for at-home blood draws, Superpower is designed for people who want clarity, convenience, and real accountability - all in one place.

Key benefits of Vitamin D, 25-Hydroxy testing

• Measures your vitamin D status to guide bone, immune, and metabolic health.
• Spots deficiency early, before bone pain, fractures, or muscle weakness appear.
• Explains fatigue, mood changes, or frequent infections linked to low vitamin D.
• Guides personalized supplementation to restore optimal levels safely and effectively.
• Tracks response to treatment, ensuring your dose achieves and maintains target range.
• Protects bone density and reduces fracture risk, especially in older adults.
• Supports fertility and healthy pregnancy by optimizing vitamin D for conception and fetal development.
• Best interpreted with calcium, PTH, and your symptoms for complete metabolic insight.

What is Vitamin D, 25-Hydroxy?

Vitamin D, 25-hydroxy (also called 25-hydroxyvitamin D or calcidiol) is the main storage form of vitamin D circulating in your bloodstream. It forms in your liver after your skin makes vitamin D from sunlight or after you consume it through food or supplements. This conversion is the first step in activating vitamin D for use in the body.

Your body's vitamin D reservoir

25-hydroxyvitamin D serves as a reservoir that your kidneys draw from to produce the active hormone form of vitamin D (calcitriol). Because it has a long half-life and reflects both dietary intake and sun exposure, it's the most reliable indicator of your overall vitamin D status.

The gatekeeper for calcium and immune health

This biomarker reveals how much raw material your body has available to regulate calcium absorption, support bone health, and modulate immune function. Adequate levels ensure your tissues can produce active vitamin D locally when and where it's needed.

More than just bone support

Beyond calcium metabolism, 25-hydroxyvitamin D influences muscle function, cardiovascular health, and cellular growth regulation throughout the body.

Why is Vitamin D, 25-Hydroxy important?

Vitamin D, 25-hydroxy is the storage form of vitamin D circulating in your blood and the most accurate measure of your body's vitamin D status. It reflects how well your skeleton, immune system, muscles, and metabolic organs are supported by this hormone-like nutrient. Levels typically range from deficient (below 20 ng/mL) to sufficient (30–50 ng/mL), with optimal health outcomes clustering in the middle to upper portion of the sufficient range.

When levels drop too low

Insufficient vitamin D impairs calcium absorption in the gut, weakening bones and raising the risk of fractures, osteomalacia in adults, and rickets in children. You may experience muscle weakness, fatigue, frequent infections, and mood changes as immune cells and brain tissue depend on adequate vitamin D signaling. Women who are pregnant or postmenopausal and older adults face heightened vulnerability to deficiency.

When levels climb too high

Excessive vitamin D, usually from over-supplementation, can lead to hypercalcemia, where too much calcium circulates in the blood. This stresses the kidneys, causes nausea, confusion, and heart rhythm disturbances, and may promote vascular calcification over time.

The long view on vitamin D

Beyond bone health, vitamin D modulates inflammation, supports cardiovascular function, and influences insulin sensitivity. Chronic deficiency is linked to autoimmune conditions, metabolic syndrome, and increased mortality risk, underscoring its role as a foundational regulator of long-term resilience.

What do my Vitamin D, 25-Hydroxy results mean?

Low vitamin D levels

Low values usually reflect insufficient sun exposure, limited dietary intake, reduced skin synthesis with aging, or impaired absorption in the gut. Vitamin D is essential for calcium absorption and bone mineralization, so deficiency can lead to weakened bones, muscle weakness, and increased fracture risk. Low levels are also associated with immune dysregulation, mood changes, and chronic inflammation. Darker skin pigmentation, obesity, and certain medications can further reduce circulating levels.

Optimal vitamin D levels

Being in range suggests adequate support for bone health, immune function, and cellular signaling across multiple organ systems. Most experts agree that optimal levels sit in the mid to upper portion of the reference range, typically above 30 ng/mL or 75 nmol/L, where bone turnover is balanced and parathyroid hormone remains appropriately suppressed.

High vitamin D levels

High values usually reflect excessive supplementation, as toxicity from sun or food alone is rare. Elevated vitamin D can increase calcium absorption beyond normal limits, leading to hypercalcemia with symptoms like nausea, confusion, kidney stones, and vascular calcification. Very high levels may also suppress parathyroid hormone excessively.

Factors that influence vitamin D results

Seasonal variation, geographic latitude, and time spent outdoors significantly affect results. Pregnancy and lactation increase demand. Kidney and liver disease impair activation of the vitamin into its active form, calcitriol.