Method: LC-MS/MS (Liquid Chromatography/Tandem Mass Spectrometry) with creatinine normalization by Jaffe Reaction (CLIA 17D0919496); not cleared or approved by the FDA. Results reported in µg/g creatinine. Not a stand-alone diagnosis; should be interpreted in clinical context.
A derived biomarker is a value that is calculated from other directly measured biomarkers rather than being measured directly in the lab.
Key benefits of N-Acetyl (3,4-Dihydroxybutyl) Cysteine (NADB) testing
- 1,3-butadiene exposure tracking
- tobacco smoke and vehicular emission burden assessment
What is N-Acetyl (3,4-Dihydroxybutyl) Cysteine (NADB)?
NADB is a urinary mercapturic acid metabolite of 1,3-butadiene, a volatile organic compound found in cigarette smoke, vehicle exhaust, and some industrial air emissions. Measured via LC-MS/MS.
Why is N-Acetyl (3,4-Dihydroxybutyl) Cysteine (NADB) important?
1,3-butadiene is classified as a known human carcinogen (per IARC Group 1 and EPA). Major non-occupational sources include tobacco smoke and high-traffic urban environments. Urinary NADB provides a personal biological measure of recent butadiene exposure.
What insights will I get?
Your NADB level may reflect cigarette smoke, heavy-traffic air exposure, or proximity to industrial emissions. Non-smokers with elevated NADB may benefit from reviewing daily commute routes and time spent in high-emission environments.





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