Method: LC-MS/MS (Liquid Chromatography/Tandem Mass Spectrometry) with creatinine normalization by Jaffe Reaction (CLIA 17D0919496); not cleared or approved by the FDA. Results reported in µg/g creatinine. Not a stand-alone diagnosis; should be interpreted in clinical context.
A derived biomarker is a value that is calculated from other directly measured biomarkers rather than being measured directly in the lab.
Key benefits of N-Acetyl (2-Cyanoethyl) Cysteine (NACE) testing
- Acrylonitrile exposure tracking
- tobacco smoke chemical burden assessment
- plastics and synthetic fiber monitoring
What is N-Acetyl (2-Cyanoethyl) Cysteine (NACE)?
NACE is a urinary metabolite of acrylonitrile, an industrial chemical used in plastics, synthetic fibers, and rubber production. Cigarette smoke is the primary non-occupational source. Measured via LC-MS/MS.
Why is N-Acetyl (2-Cyanoethyl) Cysteine (NACE) important?
Acrylonitrile is classified as a probable human carcinogen at elevated exposures (IARC Group 2A). Urinary NACE provides a direct measure of recent personal exposure; smoking - direct or secondhand - is the dominant source in most non-occupationally exposed individuals.
What insights will I get?
Your NACE level may reflect direct or secondhand tobacco smoke, contact with acrylonitrile-based synthetic materials, or occupational exposures. Serial testing before and after lifestyle changes may show measurable shifts in exposure.





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