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Folate Test

Folate Test

January 21, 2026
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Do I need a Folate test?

Feeling unusually tired, struggling with brain fog, or noticing changes in your mood? Could low folate be contributing to these symptoms?

Folate is essential for producing red blood cells, supporting brain function, and maintaining your energy levels. When folate is low, you may experience fatigue, difficulty concentrating, and mood changes that impact your daily life.

Testing your folate gives you a quick snapshot of whether deficiency is behind your symptoms, empowering you to personalize your nutrition and supplement choices. It's the first step toward addressing the root cause and reclaiming your energy and mental clarity.

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Get tested with Superpower

If you’ve been postponing blood testing for years or feel frustrated by doctor appointments and limited lab panels, you are not alone. Standard healthcare is often reactive, focusing on testing only after symptoms appear or leaving patients in the dark.

Superpower flips that approach. We give you full insight into your body with over 100 biomarkers, personalized action plans, long-term tracking, and answers to your questions, so you can stay ahead of any health issues.

With physician-reviewed results, CLIA-certified labs, and the option for at-home blood draws, Superpower is designed for people who want clarity, convenience, and real accountability - all in one place.

Key benefits of Folate testing

  • Measures folate levels to spot deficiency that can cause anemia and fatigue.
  • Flags low folate before pregnancy to protect against neural tube defects.
  • Explains symptoms like weakness, memory problems, or tingling in hands and feet.
  • Guides folate supplementation to correct deficiency and restore healthy red blood cells.
  • Tracks response to treatment and confirms levels have returned to normal range.
  • Clarifies whether anemia is due to folate or B12 deficiency for targeted care.
  • Best interpreted with vitamin B12 and complete blood count for full picture.

What is Folate?

Folate is a water-soluble B vitamin (vitamin B9) that your body cannot make on its own. You obtain it from food - especially leafy greens, legumes, and fortified grains - or from supplements. The synthetic form used in supplements and fortification is called folic acid, which your body converts into active folate.

Folate powers your cellular engine

Folate is essential for building and repairing DNA, the genetic blueprint in every cell. It also plays a central role in cell division, making it especially critical during periods of rapid growth like pregnancy, infancy, and tissue repair.

A key player in brain chemistry and blood health

Beyond DNA, folate helps produce neurotransmitters that regulate mood and cognition. It works closely with vitamin B12 to form healthy red blood cells. Without adequate folate, cells can't divide properly, leading to large, immature blood cells and a type of anemia. Measuring folate in blood reflects your recent intake and your body's current reserves.

Why is Folate important?

Folate, a B vitamin essential for DNA synthesis and cell division, powers the body's ability to build and repair tissues, produce red blood cells, and support neurological function. Every rapidly dividing cell, from bone marrow to the lining of the gut, depends on adequate folate to replicate genetic material accurately. Without it, growth stalls, blood formation falters, and the nervous system becomes vulnerable.

When folate runs low, cells can't divide properly

Insufficient folate leads to megaloblastic anemia, where red blood cells grow large and immature, causing fatigue, weakness, and pale skin. The nervous system may suffer as well, with symptoms like numbness, memory problems, and mood changes. In pregnancy, low folate sharply raises the risk of neural tube defects in the developing fetus, making adequate intake critical before conception and during the first trimester.

Elevated folate is less common but worth noting

High folate levels are unusual from diet alone and typically result from supplementation. Excessive intake can mask vitamin B12 deficiency, allowing neurological damage to progress undetected. Some research suggests very high levels may interfere with immune function, though clinical significance remains debated.

Folate connects blood, brain, and development

Folate sits at the crossroads of hematology, neurology, and reproductive health. Its role in methylation and homocysteine metabolism links it to cardiovascular risk and cognitive aging. Maintaining balanced folate status supports lifelong cellular integrity and protects the next generation.

What do my Folate results mean?

Low folate usually reflects inadequate intake or absorption

Low values usually reflect insufficient dietary intake, poor absorption in the small intestine, or increased demand that outpaces supply. Folate is essential for DNA synthesis and cell division, so deficiency impairs red blood cell production and leads to macrocytic anemia, where cells grow large but remain immature. It also disrupts nervous system function and raises homocysteine, a marker linked to vascular and cognitive risk. During pregnancy, low folate increases the risk of neural tube defects in the developing fetus. Certain medications, including methotrexate and some anticonvulsants, interfere with folate metabolism and can drive levels down.

Optimal folate supports cell division and methylation

Being in range suggests adequate folate availability for red blood cell formation, DNA repair, and methylation reactions that regulate gene expression and neurotransmitter synthesis. Optimal values typically sit in the mid to upper portion of the reference range, supporting stable homocysteine metabolism and healthy cell turnover.

High folate usually reflects supplementation or fortification

High values usually reflect recent or ongoing supplementation, consumption of fortified foods, or laboratory variation. Folate itself has low toxicity, but persistently elevated levels may mask underlying vitamin B12 deficiency, delaying diagnosis of neurological complications.

Interpretation depends on diet, supplements, and clinical context

Results vary with dietary patterns, supplement use, and certain medical conditions affecting absorption or metabolism. Pregnancy and lactation increase folate requirements substantially.

Method: FDA-cleared clinical laboratory assay performed in CLIA-certified, CAP-accredited laboratories. Used to aid clinician-directed evaluation and monitoring. Not a stand-alone diagnosis.

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Similar biomarker tests from Superpower

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Frequently Asked Questions

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How it works
What should I expect during a blood draw?
  • A trained phlebotomist will guide you through the process.
  • A tourniquet is placed on your arm, the site is cleaned, and a small needle is used to collect blood into one or more tubes.
  • Results are usually ready in about a week.
  • Most people feel only a quick pinch.
  • The needle is removed, gentle pressure is applied, and a bandage is placed.
How do I prepare for a blood draw?
  • Drink plenty of water beforehand — hydration makes veins easier to find.
  • Wear loose sleeves so your arm is easy to access.
  • Follow any fasting instructions you’ve been given.
  • Let us know if you’re on medications, have fainted before, or have needle anxiety.
What should I do after my blood draw?
  • Press gently on the site for a few minutes.
  • Keep the bandage on for 4-6 hours.
  • Skip heavy lifting or strenuous exercise for the rest of the day.
  • Drink extra water to rehydrate.
  • Monitor the site for redness, swelling, or pain.
How do I book a blood draw with Superpower?

Your membership includes:

  • An annual full body test and report across 100+ biomarkers
  • A personalized action plan to optimize your biomarkers and reach your health goals
  • A dashboard to centralize your health data and track changes across a lifetime
  • Access to a health concierge for questions on your plan and help scheduling
  • Plus a marketplace of curated health products and services cheaper than amazon

Many concierge clinics charge $10k – $100k for their services, we’ve built technology to make the world’s best healthcare as accessible as possible via an all-in-one membership.

Where can I take my blood test?

Superpower is currently available in the following US states:

  • Alabama
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  • Delaware
  • District of Columbia
  • Florida
  • Georgia
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  • Indiana
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  • Washington
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Our testing
Does Superpower replace my primary care provider?

Superpower specializes in prevention-based testing and treatments and is not intended for emergency or immediate health issues.

While you will have a Superpower care team, your annual membership is designed to complement a primary care doctor if you have one, not replace them.

We are happy to help you share any test results with an outside provider to ensure you receive well-rounded medical care.

How fast are blood test results and how do I read them?

Your annual lab test panel takes about a week to process. We will text you as soon as they become available in your dashboard. Other types of tests may have different testing windows. The Superpower concierge is your own health assistant who helps answer your questions on your results, ensure smooth scheduling, coordination of any office-based tests, specialist referrals as needed, and navigating you to interface with your care team.

Does Superpower accept health insurance?

Superpower membership and products are all eligible for HSA/FSA funding.

We see Superpower like a gym membership for those committed to prevention and performance. Superpower is a bridge between wellness and healthcare. Health insurance traditionally focuses on reactive care whereas, at Superpower, we believe it’s never too early to start looking out for your long-term health.

What if I want more than 1 blood test per year?

Absolutely — you're not limited to just one. Your membership includes one comprehensive 100+ biomarker blood test each year, but if you'd like to track your progress more closely, you can add extra tests at any time. Each additional full-panel test costs $179. You can order as many as you'd like throughout the year.

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Frequently Asked Questions about Folate Test

What is folate (vitamin B9), and how is it different from folic acid?

Folate is a water-soluble B vitamin (vitamin B9) your body needs for DNA building, cell division, and red blood cell production, but cannot make on its own. You get folate from foods like leafy greens, legumes, and fortified grains, or from supplements. Folic acid is the synthetic form used in supplements and food fortification, and your body converts it into active folate.

How does folate support DNA synthesis, cell division, and tissue repair?

Folate is essential for building and repairing DNA - the genetic blueprint inside every cell. Because it powers cell division, folate is especially important during rapid growth and repair, including pregnancy, infancy, and tissue healing. When folate is adequate, cells can replicate accurately and tissues can renew normally. When folate is low, cell division slows and body systems with fast turnover (like bone marrow) are affected first.

What are the most common symptoms of low folate levels and folate deficiency?

Low folate can cause fatigue, weakness, pallor, shortness of breath, and “brain fog” due to impaired oxygen delivery from abnormal red blood cells. It may also be linked to mood changes, memory problems, and tingling in the hands and feet. Because folate supports neurotransmitter production and blood formation, deficiency can present as cognitive and mood symptoms alongside anemia-related complaints.

How does low folate cause macrocytic or megaloblastic anemia?

Folate is required for proper DNA synthesis in developing red blood cells. When folate is insufficient, red blood cell precursors can’t divide normally, so they become large, immature cells (megaloblasts). This leads to macrocytic (megaloblastic) anemia, where red blood cells are oversized and less efficient at carrying oxygen. The result is fatigue, poor exercise tolerance, and other anemia symptoms tied to reduced oxygen delivery.

Why is folate testing important before and during early pregnancy for neural tube defect prevention?

Folate needs rise substantially in pregnancy, and adequate folate before conception and in early pregnancy helps protect fetal brain and spinal cord development. Low maternal folate is strongly associated with increased risk of neural tube defects. Folate testing can flag low levels early - before pregnancy or in the first trimester - so supplementation and dietary changes can be started promptly to support healthy fetal development.

How do I interpret low, optimal, and high folate blood test results?

Low folate usually suggests inadequate intake, poor absorption, increased demand (such as pregnancy), or interference from alcohol or certain medications, and it can lead to macrocytic anemia and neurological symptoms. Optimal folate supports normal DNA synthesis, methylation, and red blood cell maturation - often reflected by mid-to-upper reference range values. High folate is most often from supplements or fortified foods and can sometimes mask vitamin B12 deficiency.

Why do doctors check vitamin B12 and a complete blood count (CBC) with folate testing?

Folate and vitamin B12 work together to form healthy red blood cells and support nervous system function. A CBC helps detect anemia patterns (like macrocytosis), while vitamin B12 testing helps distinguish folate deficiency from B12 deficiency for targeted treatment. This matters because high folate intake can partially correct anemia while B12-related neurological damage continues. Interpreting folate alongside B12 and CBC gives a clearer clinical picture.

What causes low folate levels, including diet, alcohol use, malabsorption, and medication effects?

Low folate can be caused by low dietary intake (not enough leafy greens, legumes, or fortified grains), chronic alcohol use, and malabsorption disorders such as celiac disease. Increased demand (notably pregnancy) can also outpace intake. Certain medications can interfere with folate metabolism or absorption, including anticonvulsants and methotrexate. These factors can reduce folate availability and contribute to anemia, fatigue, and neurological symptoms.

Is high folate dangerous, and how can it mask a vitamin B12 deficiency?

High folate levels are rarely caused by disease and most often reflect supplementation or high intake of fortified foods. While excess folate is generally excreted, elevated folate can mask vitamin B12 deficiency by improving the anemia pattern without fixing the underlying B12 problem. This can delay diagnosis while neurological damage progresses. In rare situations, very high levels may be linked to unmetabolized folic acid circulating in the blood.

What’s the difference between serum folate and red blood cell (RBC) folate tests, and which reflects long-term stores?

Serum folate reflects recent folate intake and short-term status, so it can change with diet or supplements. Red blood cell (RBC) folate is considered a better indicator of longer-term tissue stores because it reflects folate levels during red blood cell formation over time. Depending on the clinical question - recent intake versus longer-term status - your clinician may use one or both tests, often alongside vitamin B12 and a CBC.

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